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Dental fluorosis is caused by ingestion of excessive fluoride during tooth development resulting in hypomineralization of the enamel and can present as white or brown intrinsic lesions. This case report describes a combination of minimally invasive techniques: microabrasion, bleaching, and resin infiltration to address brown enamel fluorosis present on the maxillary anterior teeth of a young patient. Before resin infiltration, air microabrasion was targeted at subsurface lesions located on the maxillary central and lateral incisors, and chairside bleaching was done with 37% hydrogen peroxide (Opalescence). After which, hypoplastic lesions on the buccal surfaces were etched before being treated with two sessions of resin infiltration (ICON and DMG). Following treatment, satisfactory esthetic outcomes were achieved. Accurate diagnosis, depth of lesions, and understanding the effectiveness and limitations of each technique are essential to select the appropriate type of treatment to obtain the best esthetic outcome. In conclusion, the conservative management of varying severity of dental fluorosis may require the appropriate use of combination treatment modalities, such as microabrasion, bleaching and resin infiltration, when clinically indicated, to address the treatment needs and achieve a desired outcome.

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Contemporary paradigms of dental caries management focus on the biological approaches to treating the disease and its principal symptom, the carious lesion. This narrative review traces the evolution of carious lesion management from the operative and invasive approaches of G. V. Black's era to the current period of minimally invasive biological approaches. The paper explains the rationale for adopting biological approaches to dental caries management and lists the five core principles of this management approach. The paper also details the aims, features, and the most recent evidence base for the different biological carious lesion management approaches. Based on current practice guidelines, collated clinical pathways for lesion management are also presented in the paper to aid clinicians in their decision-making. It is hoped that the biological rationale and evidence summarized in this paper will bolster the shift to modern biological carious lesion management approaches among dental practitioners.

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Background: Perforation repair materials should have excellent sealing ability and dislodgement resistance. While several materials have been employed for perforation repair, newer calcium-silicate materials, such as Biodentine and TheraCal LC, have shown promising outcomes. Aims: This study aimed to evaluate the effect of different irrigants on the dislodgement resistance of Biodentine and TheraCal LC when used for perforation repair in simulated conditions. Methods and Material: 3% sodium hypochlorite, 2% chlorhexidine gluconate, and 17% EDTA were evaluated for their effect on the dislodgement resistance of Biodentine and TheraCal LC. 48 permanent mandibular molars were selected for the study. The samples were divided into two groups: Group I – Biodentine and Group II – TheraCal LC, with 24 samples each. Statistical Analysis: The mean dislodgement resistance and standard deviation of Group I (Biodentine) and Group II (TheraCal LC) were compared and Failure pattern analysis was done. Results: Biodentine showed a significant decrease in push-out bond strength after contact with 3% NaOCl, 2% CHX, and 17% EDTA whereas, TheraCal LC showed no significant decrease in push-out bond strength after exposure to 3% NaOCl, 2% CHX, and 17% EDTA. Conclusions: Overall, TheraCal LC can be considered good perforation repair material with excellent physical and biological properties.

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Context (Background): Guided endodontics has various applications, one of which is for calcified canal negotiation. Recently, a new single-tooth template has been fabricated to overcome the drawbacks of bulky guides, which are difficult to use with rubber dam isolation. Aim: This study aimed to assess the efficacy of the novel single-tooth template for negotiation of pulp canal calcification (PCC) in three-dimensional (3D)-printed resin incisors by comparing substance loss and time taken between incisal endodontic access (IEA) and single-tooth template-guided endodontic access (SGEA). Methods: Forty-two resin incisor teeth having patent canal in the apical third were used (N = 21/group). They were subcategorized based on operator's experience into senior endodontist (SE), postgraduate (PG), and undergraduate (UG) (n = 7/operator). Canals were negotiated conventionally for IEA and using the single-tooth template for SGEA. Substance loss was calculated from the volume difference between pre- and postoperative cone-beam computed tomography scans. The time taken was also recorded. Statistical Analysis Used: Statistical analysis was performed using unpaired t-test and one-way analysis of variance test. Results: Canals were successfully negotiated in 100% and 95% of teeth in the SGEA and IEA groups, respectively. Overall substance loss and time taken were significantly lesser for SGEA for all operators (P < 0.001). In the IEA group, post hoc test showed statistical significance between SE and UG for substance loss (P < 0.05) and SE–UG and PG–UG for time taken (P < 0.05). No significant difference among operators was noted for both parameters in SGEA. Conclusions: SGEA resulted in significantly lesser substance loss and time taken for canal negotiation in 3D-printed resin incisors with simulated PCC. This was independent of the experience levels of the operator.

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Aim: Using confocal laser scanning microscopy (CLSM), the current study assessed the impact of nonthermal atmospheric plasma (NTAP) on the dentinal tubule penetration of bioceramic and epoxy resin-based root canal sealers (CLSM). Materials and Methods: Forty human mandibular premolar teeth with a single root that had just undergone extraction were chosen and biomechanical preparation of root canals was done with ProTaper Gold rotary Nickel-titanium instruments. Samples were divided into four groups (n = 10). Group 1: Bioceramic sealer (BioRoot RCS); Group 2: Epoxy resin-based sealer (AH Plus) without application of NTAP; Group 3: Bioceramic sealer (BioRoot RCS); and Group 4: Epoxy resin-based sealer (AH Plus) with the application of NTAP for 30 s. In Groups 3 and 4, all of the samples underwent obturation with the appropriate sealers following NTAP application. For determination of the sealer's dentinal tubule penetration values, slices with a thickness of 2 mm were taken from the middle third of sample's root and examined using CLSM. The acquired data were statistically analyzed with one-way analysis of variance and the Post hoc Tukey's test. The cutoff for statistical significance was P < 0.05. Results: In comparison to other groups, the maximum sealer penetration values into dentinal tubules were significantly higher in Group 3 for Bioceramic sealer with NTAP application and Group 4 for Epoxy resin-based sealer with NTAP application. Conclusion: NTAP application increased the dentinal tubule penetration of bioceramic and epoxy resin-based sealers in comparison to groups without NTAP application.

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Aim: Four bonding agents and a composite restorative resin were evaluated in patients having noncarious cervical defects. Materials and Methods: This clinical trial was conducted in patients having at least 4 noncarious cervical defects in posterior teeth evaluating the clinical effectiveness in relation to retention, discoloration at margins, and postoperative sensitivity of 5^th, 6^th, 7^th, and 8^th generations of bonding agents over a period of baseline, 3, 6, 12, and 24 months. Statistical Analysis: Data were recorded and put into statistical analysis using Chi-square tests. Results: At 24 months, retention rate was found out to be 92.6% for the 7^th generation which was better than 5^th (66.7%) and 6^th (70.4%) generation whereas significant marginal discoloration was seen at 6 months follow-up in which 5^th generation showed maximum results. However, all the four generations have an equal score of postoperative sensitivity at all the time intervals. Conclusions: The 7^th generation adhesives performed better than other generations in terms of retention. Changes in marginal discoloration were detected at 6 months with maximum score in 5^th generation adhesives.

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Background: Considering the potential of translucent zirconia for application in esthetic restorations, it is necessary to find effective methods with the least adverse effects to increase its bond strength to resin cement. Aims: This study aimed to test if different conservative surface treatments and cement types could affect the micro-shear bond strength (μSBS), failure mode, and bonding interface between resin cement and translucent zirconia. Materials and Methods: In this in vitro experimental study, translucent zirconia blocks were divided into four groups based on the surface treatment they received: no treatment, argon plasma, primer (Pr), and Pr + plasma. Each group was further divided into two subgroups based on the applied cement: PANAVIA F2 and Duo-Link cement. Fourteen cement columns with a diameter of 1 mm were placed on each block (n = 14); all the specimens were immersed in 37°C water for 24 h. Afterward, μSBS was evaluated (P < 0.05), and the mode of failure was determined by a stereomicroscope (×10). The cement–zirconia interface and the surface hydrophilicity (contact angle) were also evaluated. Statistical Analysis: Two-way analysis of variance (ANOVA) was used to evaluate the effect of surface preparation, cement types, and incubator, simultaneously (P < 0.05). The bond strengths after incubation were analyzed by one-way ANOVA (P < 0.05). Failure mode, contact angle, and cement–zirconia interface were analyzed descriptively. Results: The highest bond strength was seen in Pr surface treatment for Duo-Link cement; however, this group was not significantly different from Pr and PANAVIA F2 cement and Pr + plasma and Duo-Link cement (P = 0.075) groups. All plasma specimens in the incubator failed prematurely. The mode of failure in all specimens was adhesive. The lowest and highest contact angles were seen in Pr + plasma and the control groups, respectively. Conclusion: The use of Pr could successfully improve the bond strength of resin cement to translucent zirconia while plasma was not an acceptable and durable substitute.

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One of dentistry's primary objectives is the long-term maintenance of teeth. Hemisection may be the best line of action when only one root is decayed and the other one is intact. This case report outlines and depicts a situation in which the deteriorated terminal abutment was part of a cantilevered fixed prosthesis. Hemisection and prosthesis rehabilitation had successful outcomes.

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Aim: The aim of this study was to assess and compare the quantity of apically debris which was extruded apically by TruNatomy (TN), ProTaper Next (PTN), HyFlex electric discharge machining (EDM), and HyFlex controlled memory (CM), following root canal preparation. Materials and Methods: Sixty extracted single-canal mandibular premolars were used. The root canal preparation was done with TN, HyFlex EDM, PTN, or HyFlex CM files. The preweight debris, which was extruded apically, was collected in the Eppendorf tube and later on incubated at 670°C for 3 days and weighed again to record the extruded debris. Results: The result showed that there was a significant reduction in debris extrusion by TN system, followed by PTN system, HyFlex EDM, and maximum extrusion in HyFlex CM (P < 0.05). Between the PTN and TN groups as well as between the HyFlex EDM and HyFlex CM groups, statistically significant difference was not observed (P > 0.05). Conclusion: Apical debris extrusion is the inherent nature of the all file systems. Nevertheless, the TN file system produced substantially minimum debris extrusion among other systems compared in the study.

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Introduction: The objective of this in vitro study was to examine the impact of different endodontic chelating agents on the flexural strength and microhardness of root dentin. Materials and Methods: Fourty dentin sticks of (1 mm × 1 mm × 12 mm) were obtained from 10 single-rooted premolars and divided into four groups (n = 10). One stick from each tooth was assigned to one of the experimental groups and was soaked in one of the experimental chelating solutions for 5 min 17% ethylenediaminetetraacetic acid (EDTA), 2.5% phytic acid (PA), 18% etidronic acid, or saline (control group). Following the 5-min soak, the sticks' flexural strength was evaluated using a 3-point loading test using the universal testing machine, and the surface microhardness was tested using a Vickers's microhardness tester. Results: PA (2.5%) and etidronic acid (18%) showed no significant detrimental effect on either the flexural strength or the surface microhardness of radicular dentin compared to the control. EDTA (17%) exhibited a significant drop in the flexural strength and microhardness of radicular dentin compared to the other groups. Conclusions: PA and etidronic acid chelators do not compromise the surface and bulk mechanical properties of radicular dentin.

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Context: Elucidating the effects of leachates from composite resins (CRs) on cells by examining the transcription level of detoxification genes and the antioxidant-responsive element (ARE), would be helpful in clinical practice. Aims: The aim of the study is to investigate the cytotoxicity of commercially available CRs, we used a reporter assay system to evaluate intracellular stress based on ARE-mediated transcription. Setting and Design: The study design was an in vitro study. Materials and Methods: Seven kinds of CRs were each placed in four-well plates to which culture medium was added and then light-cured. The prepared samples were used either immediately (sample A) or after incubation at 37°C for 24 h (sample B) in the subsequent ARE-luciferase reporter assay, in which HepG2 cells stably expressing an ARE-regulated luciferase reporter gene (HepG2-AD13 cells) were cultured for 6 h in culture media with the CR eluate (samples A or B) or without (control) (n = 4). In the cell viability assay, cell viability in various solutions with the same incubation time was confirmed by MTT assay (n = 4). Statistical analysis was performed using the paired t-test and one-way analysis of variance. Results: All CR solutions showed an increase in ARE activation rate; a CR with spherical nanofillers showed the highest ARE activation rate of 108.5-fold in sample A. Cell viability was not significantly reduced for any of the CRs in sample A. However, the CR-containing bisphenol A-glycidyl methacrylate (Bis-GMA) caused a significant decrease in cell viability in sample B. Conclusions: The intracellular stress in the viable cells differed among the CRs, depending on the type of monomer used. In particular, Bis-GMA-containing hydroxyl groups showed high cytotoxicity.

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Maxillary central incisor is considered the tooth with least anatomical variations. In literature, the prevalence of single root and single canal in maxillary central incisor has been reported as 100%. Only a handful of case reports suggesting more than one root or one canal are available and are mostly associated with developmental anomalies such as gemination and fusion. This article describes a rare case report of retreatment of a maxillary central incisor with two roots with normal clinical crown which was confirmed by cone-beam computer tomography (CBCT). A 50-year-old Indian male patient presented with pain and discomfort on a root canal-treated anterior tooth. Pulp sensibility testing of left maxillary central incisor was negative. Intraoral periapical digital radiograph revealed an obturated canal with suspected outline of a second root which got confirmed with cone shift technique. The tooth was treated under dental operating microscope during which two canals were located and retreatment was completed. Postobturation, CBCT was performed to study the root and canal morphology. Clinically and radiographically, all the follow-up examinations revealed an asymptomatic tooth without any active periapical lesion. The present case report emphasizes the fact that clinicians should approach each case with an open mind having a thorough knowledge of the normal tooth anatomy and should suspect variations in every case to ensure successful endodontic outcome.

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Background: Determination of working length (WL) is necessary for the successful outcome of root canal treatment (RCT). Common methods in WL determination include tactile, radiographic, and electronic apex locators (EAL). Aim: The aim of this study was to compare three methods of WL determination to the actual visualization of the apical constriction (AC). Materials and Methods: Consecutive patients with indications for extraction of single-rooted single canal teeth at the University of Ghana Dental School clinic were randomly assigned to three groups. In-vivo root canal WL was determined by tactile sensation, digital radiography, and a 5^th generation EAL (Sendoline S5). Files were cemented in the canals after the in-vivo measurements. The apical 4–5 mm of the roots was trimmed to expose the inserted files and the AC. Actual WL, as determined by visualization of the AC, was done using a digital microscope. Different WLs were then compared for the various groups, and the mean actual canal lengths were reported. Results: EAL accurately predicted the AC in 31 (96.9%) teeth, while the digital radiographic and tactile sensation methods accurately predicted the constriction in 19 (59.4%) and 8 (25%) teeth, respectively, in the study population. The mean working canal lengths for single-rooted teeth showed no observable difference among sexes, age categories, and side of the jaw. Conclusion: The EAL provided more reliable and accurate WL measurements for single-rooted teeth among Ghanaians, compared to digital radiography and tactile methods.

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Objectives: The objective of the study was to evaluate and compare the surface topographies of WaveOne Gold (WOG), FlexiCON X1, and EdgeOne Fire (EOF) reciprocating files before and after root canal instrumentation using different irrigating solutions. Materials and Methods: Forty-eight extracted mandibular molars were selected and randomly assigned into three groups (n = 16) based on the file system used and each group was divided into two subgroups based on the irrigants used during root canal treatment. (Group-1: WOG; Group-2: FlexiCON X1; Group-3: EOF; Subgroup-A: 3% sodium hypochlorite [NaOCl] +17% ethylenediaminetetraacetic acid [EDTA]; Subgroup-B: Citra wash as irrigating solutions). The surface topography of the files was analyzed using atomic force microscope before and after instrumentation. Average roughness and root mean square roughness were calculated. Independent and paired t-tests and one-way analysis of variance with Tukey's post hoc were used for statistical analysis. Results: Atomic force microscopy results showed an increase in surface roughness after instrumentation, EOF showing the highest roughness. Citra wash showed more roughness compared to NaOCl and EDTA together. However, the surface roughness between the experimental groups WOG and EOF are statistically insignificant and this insignificance was also seen among subgroups (P>0.05). Conclusion: Instrumentation with various irrigating solutions affected the surface topography of EOF, WOG, and FlexiCON X1 reciprocating files.

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Introduction: The study evaluated the influence of coronal restoration after pulpotomy on the strength of electrical stimulus reaching the radicular pulp using an electric pulp test (EPT). Materials and Methods: The pulp tissue from ten freshly extracted mandibular premolar teeth was removed and replaced with an electroconductive gel. The cathode probe of Powerlab was inserted into the pulp space and the anode probe was attached to the EPT handpiece. The EPT probe coated with electro-conducting material was positioned in the middle third of the buccal crown surface. The EPT stimulus reaching the pulp space of an intact tooth at 40 numerical readings was recorded. The tooth was removed from model and endodontic access was made. The 2-mm thick mineral trioxide aggregate was placed at the cementoenamel junction followed by composite resin restoration. The experimental setup was re-established and postpulpotomy EPT stimulus data were recorded. The data collected were compared using the Wilcoxon signed-rank test. Results: There was a statistically significant difference (P = 0.038) between observed between the strength of EPT stimulus reaching the pulp space in prepulpotomy (mean 91.18 ± 101.02 V and median 25.79 V) and postpulpotomy (mean 58.49 ± 77.13 V and median 13.75 V) tooth samples. Conclusion: The placement of the restoration and pulp capping agent after pulpotomy dampens the strength of EPT stimulus reaching the pulp canal space.

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Aims: The aim of the study is to compare the dissolution effectiveness of xylene, thyme oil, and orange oil on three different endodontic sealers. Materials and Methods: Standardized stainless steel molds were used to prepare 210 samples (70 for each endodontic sealer). The samples were divided into three groups based on sealers. Three experimental groups with 20 samples each were immersed in organic solvents. One control group of 10 samples was immersed in distilled water. Each group was further subdivided into two subgroups based on immersion time (2 and 10 min). Inferential statistics included one-way ANOVA, post hoc Tukey, and paired t-test. Results: Thyme showed significantly more dissolution capacity at 10 min compared to 2 min in dissolving AH Plus sealer whereas this difference was nonsignificant for Roekoseal and MTA Fillapex. Orange oil showed significantly more dissolution at 10 min compared to 2 min in dissolving AH Plus sealer and Roekoseal whereas this difference was nonsignificant concerning MTA Fillapex. Xylene showed significantly more dissolution capacity at 10 min compared to 2 min in dissolving AH Plus sealer, Roekoseal, and MTA Fillapex. Conclusions: Among the three solvents, xylene showed the highest dissolution of all three sealers. Orange oil was superior to thyme oil in dissolving the sealers. All the sealers showed more dissolution in all the solvents at 10 min compared to 2 min.

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Context: The purpose of this study was to evaluate and compare the centering ability and canal transportation of TruNatomy, OneCurve, and Jizai file systems to assess their performance in oval-shaped canals using cone-beam computed tomography imaging. Materials and Methods: Forty-two fully formed single-rooted mandibular premolars were selected with a buccolingual canal size 2–2.5 times the mesiodistal size at 5 mm from the apex, with 0°–10° canal curvature with a 5–6 mm radius, at 5 mm from the apex. The teeth were divided into three groups (n = 14) and prepared with TruNatomy, OneCurve, and Jizai files based on the manufacturer's instructions. Cone-beam computed tomographic images were taken before and after instrumentation. The canal transportation and centering ability was calculated at 3, 6, and 9 mm from the apex in both mesiodistal and buccolingual directions. Statistical Analysis: Intergroup comparison was done using Kolmogorov–Smirnov test. Intragroup comparison was done using Freidman test. A comparison of categorical variables was done using the Chi-square test. Results: The results obtained did not present any statistically significant difference between the three groups, with TruNatomy and OneCurve showing relatively lesser canal transportation and better centering ratio when compared to the Jizai file system. Conclusions: It can, therefore, be concluded that all three systems used in the study are capable of safely preparing root canals with minimal errors.

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Background: Optimal biomechanical preparation, thorough irrigation, proper disinfection, and finally, well-sealed obturation is a must to attain definitive success in root canal procedure. Proper preparation of the root canal is all the more imperative to establish a hermetic apical seal with adequately placed filling materials. The present study was conducted for comparing the Cleaning Efficiency of F360 system and the WaveOne Gold (WOG) system of rotary NiTi instrumentation for a root canal. Materials and Methods: One hundred freshly extracted noncarious mandibular canines were obtained. Access cavity of standard size was made followed by the establishment of working length. Afterward, random division of all the specimens was done into two study groups: group A: the group in which F360 system was used for instrumentation and group B: the group in which the WOG system was used for instrumentation. Irrigations of all the specimens of both the study groups were done followed by root canal shaping by the instrument system of their respective study groups. Scanning electron microscope (SEM) was used for assessment after specimens were cut buccolingually. Debris score and residual smear layer score were used for assessment. Results: Among group A specimens, the mean smear layer score at the coronal third, middle third, and apical third was 1.76, 2.39, and 2.65, respectively. Among group B specimens, the mean smear layer score at the coronal third, middle third, and apical third was 1.34, 1.59, and 1.92, respectively. While analyzing statistically, it was seen that the mean smear layer score was significantly higher among specimens of group A in comparison to group B. While analyzing statistically, it was seen that the mean debris score was significantly higher among specimens of group A in comparison to group B. Conclusion: The cleaning effectiveness of WOG instruments was significantly enhanced in comparison to F360 equipment.

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Aim: The aim of the study was to evaluate the canal transportation and centering ability of rotary and reciprocating file systems using cone-beam computed tomography. Materials and Methods: Mesiobuccal canals of sixty mandibular molars were selected for the study. Canals of length 19 mm, curvature of 10°–12°, and uncalcified with fully formed apex were selected. Canals were randomly divided into three groups of 20 teeth, and canal preparation with the WaveOne Gold, TruNatomy, and One Curve systems was performed according to the manufacturers' instructions. Cone-beam computed tomographic images were taken before and after instrumentation in the same position for comparative analysis. Statistical Analysis Used: Apical transportation was calculated at the distances of 2, 3, and 4 mm from the apex. Tukey's post hoc test and unpaired “t”-tests were used to statistically analyze the data. Results: WaveOne Gold caused less canal transportation and better-centering ability than TruNatomy and One Curve at all the three levels; there was a significant difference in canal transportation and centering ability among all the groups as well as all the three levels, i.e., 2, 3, and 4 mm from the apex. Conclusion: WaveOne Gold (Reciprocating) reported less canal transportation and better-centering ability than rotary instruments TruNatomy and One Curve (Rotary) at all the three levels.

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Aim: The aim of this study was to assess the impact of nonthermal atmospheric plasma (NTAP) on the bond strength of composite resin following plasma application at different steps of dentin bonding in total-etch and self-etch adhesive systems. Materials and Methods: Ninety extracted third molars were taken, and the occlusal surfaces were removed until the exposure of the dentin. Samples were distributed into two main groups: Group T: total-etch adhesive system and Group S: self-etch adhesive system. Groups are further subdivided (n = 10) based on plasma application at different steps of dentin bonding. T1: surface etching with 37% phosphoric acid and bonding agent application. T2: plasma application and bonding agent application. T3: plasma application, etching, and bonding agent application. T4: etching, plasma application, and bonding agent application. T5: etching, plasma application, bonding agent application, and again plasma application. S1: self-etch bonding agent application. S2: plasma application and bonding agent application. S3: Bonding agent application and plasma application. S4: plasma application, bonding agent application, and again plasma application. For all the samples, composite resin buildup was done, and shear bond strength (SBS) was measured. The contact angle was measured at different steps of dental adhesive systems. Statistical Analysis: Two-way analysis of variance and the post hoc Tukey's test were used for analysis, regarding P < 0.05 as statistically significant. Results: Among all groups of total-etch and self-etch adhesives, Group T4 (48.81 Mpa) and Group S2 (36.59 Mpa), respectively, have demonstrated significantly greater bond strength values than the corresponding control groups. Conclusion: NTAP enhanced the composite resin's SBS when plasma treatment was done before bonding agent application and significantly reduced the contact angles of the distilled water.

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