Year : 2015 | Volume
: 18 | Issue : 1 | Page : 51--55
Effect of epigallocatechin-3-gallate application for remaining carious dentin disinfection
Jorgiana Silva de Assis, Ramille Araujio Lima, Juliana Paiva Marques Lima, Lidiany Karle Azevedo Rodrigues, Sï¿½rgio Lima Santiago
Faculty of Pharmacy, Dentistry and Nursing, Department of Restorative Dentistry, Federal University of Cearï¿½, Fortaleza, Cearï¿½, Brazil
Context: Epigallocatechin-3-gallate (EGCG) is a flavonoid extracted from green tea that demonstrated antimicrobial activity.
Aims: To evaluate the efficacy of EGCG 0.5%, 1%, and 2% concentrations as an antimicrobial solution in dentin caries-like lesions induced in a bacterial-based in vitro model.
Materials and Methods: Twenty-five human dentin specimens were submitted to a microbial-based caries model by immersion in brain heart infusion (BHI) broth inoculated with Streptococcus mutans UA159, for 5 days. After the demineralization period, the specimens were randomly divided into groups: Group I: 0.9% saline solution; Group II: 2% chlorhexidine digluconate; Group III: 0.5% EGCG; Group IV: 1% EGCG; and Group V: 2% EGCG. After the treatments, carious dentin samples were harvested from dentin specimens and analyzed by colony-forming unit (CFU) counts. Data were analyzed by ANOVA and Tukeyï¿½SQï¿½s test.
Results: Log reduction values (SD, CFU.mg -1 ) for Groups I-V were: 5.02 (0.16), 3.96 (0.43), 4.74 (0.26), 4.89 (0.56), and 4.91 (0.40), respectively. There was no statistical difference between the EGCG concentrations and saline solution (P > 0.05). Furthermore, there was no statistical difference between EGCG concentrations (P > 0.05). However, there was a statistically significant difference between the chlorhexidine digluconate group and the other groups (P < 0.05).
Conclusion: EGCG at the studied concentrations were not effective in eliminating S. mutans from dentin caries-like lesions.
Prof. Sï¿½rgio Lima Santiago
Rua Monsenhor Furtado, s/n Rodolfo Teï¿½filo, 60.430-355 Fortaleza-CE
|How to cite this article:|
de Assis JS, Lima RA, Marques Lima JP, Azevedo Rodrigues LK, Santiago SL. Effect of epigallocatechin-3-gallate application for remaining carious dentin disinfection.J Conserv Dent 2015;18:51-55
|How to cite this URL:|
de Assis JS, Lima RA, Marques Lima JP, Azevedo Rodrigues LK, Santiago SL. Effect of epigallocatechin-3-gallate application for remaining carious dentin disinfection. J Conserv Dent [serial online] 2015 [cited 2023 Dec 6 ];18:51-55
Available from: https://www.jcd.org.in/article.asp?issn=0972-0707;year=2015;volume=18;issue=1;spage=51;epage=55;aulast=de;type=0