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| Year : 2021 | Volume
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| Issue : 2 | Page : 130-134 |
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| The effect of natural reducing agents on push-out bond strength of AH plus and BioRoot RCS to sodium hypochlorite treated root dentin |
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S Mann Navjot1, Jhamb Ashu2, Kaur Kamalpreet3, K Mann Navneet4, Rana Manu2, Batra Divya5
1 Professor and Head, Department of Conservative Dentistry and Endodontics, National Dental College and Hospital, Punjab, India
2 Reader, Department of Conservative Dentistry and Endodontics, National Dental College and Hospital, Punjab, India
3 PG Student, Department of Conservative Dentistry and Endodontics, National Dental College and Hospital, Punjab, India
4 Reader, Department of Prosthodontics, National Dental College and Hospital, Punjab, India
5 Senior Lecturer, Department of Conservative Dentistry and Endodontics, National Dental College and Hospital, Punjab, India
Click here for correspondence address and email
| Date of Submission | 26-Jan-2021 |
| Date of Decision | 04-Feb-2021 |
| Date of Acceptance | 23-Mar-2021 |
| Date of Web Publication | 09-Oct-2021 |
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 Abstract | | |
Aims: To evaluate the effect of natural anticoagulants 6.5% proanthocyanidin (PA) and 25% bamboo salt on push-out bond strength (PBS) of AH Plus and BioRoot RCS to dentin.
Subjects and Methods: 30 single-rooted extracted human teeth were collected. After establishing the working length samples were prepared up to size F3. 5 ml of 3% NaOCl was used as irrigant during instrumentation followed by rinse with 5 ml of 17% ethylenediaminetetraacetic acid. Samples were randomly divided into groups based on the final irrigation solution: Group I – AH plus sealer group, Ia – Saline group, Ib – PA group, Ic – Bamboo salt (BS) group. Group II – BioRoot RCS group, IIa – Saline group, IIb – PA group, IIc – BS group. After obturation, samples were embedded in self-cure acrylic resin and 2 mm thick root slices were made at coronal middle and apical 3rd. These slices were subjected to PBS testing followed by stereomicroscopic examination for checking the mode of failure.
Statistical Analysis Used: Kruskal–Wallis and Dunn's post hoc test.
Results: 3% NaOCl significantly decreased the bond strength of AH Plus as compared to BioRoot RCS to dentin (P < 0.05). Both PA and BS were capable of increasing the PBS of AH Plus and BioRoot RCS to NaOCl-treated dentin.
Conclusions: Final irrigation with antioxidants such as PA and BS eliminates the risk of reduced bond strength of sealer to root canal walls, which ensues following the use of NaOCl as an irrigant.
Keywords: Antioxidants; bamboo salt; calcium silicate; epoxy resin; free radicals; proanthocyanidin; pushout bond strength
How to cite this article:
Navjot S M, Ashu J, Kamalpreet K, Navneet K M, Manu R, Divya B. The effect of natural reducing agents on push-out bond strength of AH plus and BioRoot RCS to sodium hypochlorite treated root dentin. J Conserv Dent 2021;24:130-4 |
How to cite this URL:
Navjot S M, Ashu J, Kamalpreet K, Navneet K M, Manu R, Divya B. The effect of natural reducing agents on push-out bond strength of AH plus and BioRoot RCS to sodium hypochlorite treated root dentin. J Conserv Dent [serial online] 2021 [cited 2023 Nov 28];24:130-4. Available from: https://www.jcd.org.in/text.asp?2021/24/2/130/327844 |
| Introduction | |  |
The purpose of endodontic therapy is to ensure a fluid tight seal thereby creating a single block configuration which seals the canal space hermetically. Sealers are used to create a seal between the core material and dentinal walls. It should fill imperfections and increase the adaptation of the root filling material to the canal walls.[1],[2]
Adhesion between the root dentin, core material, and the sealer is a desirable outcome of root canal obturation.[2] This aids in reducing coronal and apical leakage, thereby improving the health of the periapical tissues.[3] Cleaning and shaping of the root canal involve the use of chemical substances to disinfect the canal as well as to remove the smear layer, to make it more receptive to the obturating material. Sodium hypochlorite (NaOCl) is a widely used root canal disinfectant. It is effective in dissolving the organic component of the smear layer.[4]
The disadvantages of NaOCl include toxicity,[5] promotion of structural changes in organic dentin components (mainly collagen),[6] and its effects on the mechanical properties of dentin, such as reduced flexural strength and the elastic modulus.[7],[8]
AH Plus (Dentsply DeTrey, Konstanz, Germany) is a hydrophobic, epoxy resin-based sealer that is widely used because of its particular physical properties.[9] The adhesiveness of AH Plus to root dentin is based on covalent bonding between the open epoxide ring and the exposed side-chain amine groups of the collagen network.[10] Dentin surface treatments with different irrigation protocols predominantly cause alterations to the collagen fibrils of dentin, which may compromise the adhesiveness of endodontic sealers to the dentin surfaces. Many studies have demonstrated that NaOCl reduces the bond strength between adhesive materials and dentin.[11],[12],[13] Furthermore, it potentially reduces the bonding strength of AH Plus sealer.[14],[15] This may be attributable to the presence of residual oxygen species on the dentin, which affects the setting time of the adhesive material.[15]
BioRoot RCS (Septodont, St. Maur-des-Fossés, France) is a calcium silicate-based sealer with a good biocompatibility.[16] The release of calcium hydroxide after hydration and the contact with phosphate from tissue fluids leads to the precipitation of calcium phosphate or calcium carbonate on the surface.[17],[18] The formation of hydroxyapatite on BioRoot RCS after contact with phosphate-buffered saline solution was reported.[18] Calcium silicates form an interfacial layer at the dentin called the “mineral infiltration zone” with increased mineralization.[19],[20]
The compromised bond strength of NaOCl-treated dentin could be restored by the application of antioxidants before the adhesive procedure;[21] these agents interact with the by-products of NaOCl,[21] resulting in the neutralization and reversal of the oxidizing effects of NaOCl on the dentin surface.[21] Sodium ascorbate and proanthocyanidins (PA) are some of the most researched antioxidants in dentistry.[21] Naturally derived reducing agents have the ability to chemically modify collagen without damaging biological tissues and moreover, improve dentin matrix properties.[22],[23],[24],[25] The application of these provided redox potential as reducing agents for NaOCl-treated dentin, and can potentially improve the quality of collagen modified by NaOCl.
The free radical scavenging ability of PA is well-documented and is shown to be more effective than sodium ascorbate.[26] PAs are naturally occurring plant metabolites, which are a part of the group of polyphenolic compounds known as flavonoids, widely present in grape seed, cranberries, leaves of bilberry and birch, and bark of several trees.[26] PAs from grape seed extract (GSE) are composed primarily of 36% oligomeric and polymeric procyanidins, namely, catechin or epicatechin and <5% flavan-3-ol monomeric catechins.[27]
Bamboo salt (BS) is one such natural product with antimicrobial, antioxidant, and anti-inflammatory properties. It is obtained by packing and repeatedly (nine times) roasting sun-dried salt, in bamboo trunks sealed by yellow soil, using pinewood and pine resin as fuel. BS contains a wide array of macro- and micro-nutrients (B, Fe, Sr, Cu, V, and Mn) and trace elements. It is composed of 29.3% Na, 1.4% K, 0.4% S, 0.1% Ca, and 0.08% Mg as macronutrients.[28] It finds therapeutic application in the prevention and treatment of various diseases such as inflammation, allergies, gastritis, circulatory disorders, and cancers.[29],[30],[31],[32] It has been found to be as efficacious as PA in reinstating the reduced bond strength of AH Plus to NaOCl-treated dentin.[33]
Hence, the aim of this in vitro study was to determine the effectiveness of natural anticoagulants 25% Bamboo Salt and 6.5% PA, on the push-out bond strength (PBS) of AH Plus and BioRoot RCS to root canal dentin irrigated with 3% NaOCl. The null hypothesis was that neither the antioxidant agent applied nor the different regions of the canal (coronal, middle, or apical) would influence the PBS of the sealers to dentin.
| Subjects and Methods | | |
Preparation of 6.5% proanthocyanidin solution
About 6.5 g of GSE in the form of powder (Medlife Essentials, Medlife Wellness Retail Private Limited, Bengaluru, India) was collected from the capsules and dissolved in 100 ml of distilled water to make 6.5% PA solution.
Preparation of 25% bamboo salt solution
About 25 g of Bamboo Salt (GOKOCO, Korea) was dissolved in 100 ml of distilled water to make 25% BS solution.
Sample preparation
Thirty-three teeth extracted for periodontal and/or orthodontic reasons with single roots and single canals were collected and stored in distilled water until use. The teeth were decoronated at the cementoenamel junction and standardized to a length of 12 mm with the help of a low-speed diamond disk under water spray. Patency of each root canal was checked using a K-file (#15) (Mani Inc., Tochigi, Japan) and working length was established 1 mm short of the apex. Cleaning and shaping were performed by crown-down technique, using ProTaper rotary shaping and finishing files (Dentsply, Maillefer, Ballaigues, Switzerland), up to size F3. During the preparation of the canal, a total of 5 ml of 3% NaOCl (Septodont Healthcare India Pvt., Ltd., Panvel, India) was used for irrigation between instruments. After instrumentation, a final rinse with 5 ml of 17% ethylenediaminetetraacetic acid (Canalarge, Ammdent, Mohali, India) was done to remove the smear layer. Finally, the canals were irrigated with 5 ml of 3% NaOCl. The samples were divided into two groups (n = 15) based on the type of sealer used for obturation: Group 1 (AH Plus sealer), Group 2 (BioRoot RCS). They are further divided into three subgroups each based on the final treatment protocol: Group 1a (saline), Group 1b (6.5% PA), Group 1c (25% Bamboo Salt), Group 2a (saline), Group 2b (6.5% PA), and Group 2c (25% bamboo Salt). The canals were flushed with their respective solutions, keeping a standardized quantity and contact time of 5 ml and 5 min, respectively. The root canals of the samples in Groups 1b, 1c, 2b, and 2c were additionally rinsed with 5 ml of distilled water and the canals of all the samples were dried with paper points (Dentsply, Maillefer, Ballaigues, Switzerland). The canals were coated with their respective sealers with the aid of a lentulospiral (Dentsply DeTrey, Konstanz, Germany) and obturated using #F3 gutta-percha (Dentsply DeTrey, Konstanz, Germany). The root samples were coronally sealed with intermediate restorative material (IRM, Dentsply DeTrey, Konstanz, Germany) and were stored in distilled water for 7 days.
Pushout bond strength testing
A cylindrical mold was filled with autopolymerizing acrylic resin and the roots were vertically embedded in it, until the acrylic resin set. Acrylic blocks were removed from the mold, and 2 mm thick slice was obtained from the coronal, middle, and apical third of each embedded root sample using a rotating diamond disk under water-cooling. Each slice was subjected to PBS test in a universal testing machine (Tecsol, Chennai, India) using a metallic indentor with a round cross-section and diameters of 0.5 mm, 0.7 mm, and 0.9 mm customized to test slices from the apical, middle, and coronal thirds, respectively. A crosshead speed of 1 mm/min was used. The fractured samples were viewed under a stereomicroscope at ×40 magnification to analyze the mode of failure. The classification of failure modes was adopted from Jainaen et al.[34]
Data and statistical analysis
The data were tabulated and statistically analyzed using SPSS Statistics V22.0 (IBM, USA). The PBS data were subjected to Kruskal–Wallis and Dunn's post hoc test. The significance was set at P < 0.05.
| Results | | |
The mean and standard deviation of the PBS of all the groups at three different levels of the canal (in MPa) is given in [Table 1]. The PBS of samples in Group 1a, 2a was the least at all three levels. 6.5% PA (Group 1b, 2b) and 25% BS (Group 1c, 2c) treated samples showed significantly higher bond strength values compared to Groups 1a and Group 2a (P < 0.05). There was a significant difference between Groups 1 and 2 (P > 0.05). The PBS values were significantly lesser at the apical third, compared to coronal and middle thirds of the root (P < 0.05). The failure modes of the samples are given in [Table 2]. Adhesive failure at the dentin-sealer interface was the predominant mode of failure in Group 1a, whereas in Groups 1b, 1c, 2a, 2b, and 2c, mixed failures were mostly seen. | Table 1: Mean±standard deviation of the push out bond strength of all the groups at different levels of the canal (MPa)
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| Discussion | | |
The aim of endodontic therapy is not only to eliminate microorganisms by cleaning and shaping the root canal but also to ensure that the root canal system is fluid-free and that a single block configuration is created that seals hermetically the canal space. Because of the poor adhesiveness of gutta-percha, the use of sealers has been considered mandatory.
The major function of a root canal sealer is to fill imperfections and increase adaptation of the root filling material to the canal walls, failing which the chances of leakage and failure increase.[35],[36] Improved bond strength minimizes the risk of filling detachment from dentin during restorative procedures or the masticatory function, ensuring that seal is maintained and consequently, clinical success of endodontic treatment.[37]
The bond (sealer and canal walls) through frictional retention or micromechanical adhesion may be beneficial in maintaining the integrity of this crucial interface between dentine and cement. The force is applied in apico-coronal direction to avoid interference due to canal taper, during dislodgement of the filling material.[38]
Leakage studies have drawbacks, as does the micro-tensile method, which can result in premature bond failure when cutting the specimens so, in this study, a PBS test was used.[39]
The ability of the push-out test to evaluate the bonding strength surpasses that of other tests because it generates parallel fractures in the interfacial area of dentin bonding. However, a limitation of the push-out test is that it creates nonuniform stress distribution.[40] We prevented this limitation in this study by using 2 mm thick slices.
In the present study, Group 2 (BioRoot RCS) showed the higher bond strength with a statistically significant difference (P < 0.05) than Group 1 (AH Plus) irrespective of the irrigant used. This may be due to its true self-adhesive nature, which forms a chemical bond (through the production of HA during setting) with dentine.
The use of NaOCl decreased the bond strength of AH Plus to dentin more as compare to BioRoot RCS. The results of the present study are in accordance with the previous studies.[41],[42],[43] The use of NaOCl during instrumentation removes the organic debris from the canal. In due course, it also causes degradation of root dentin collagen. On contact with organic matter in the root canal, NaOCl breaks down into chloramines and protein-derived radical intermediates.[44] Daumer et al. observed that these breakdown products are capable of having an adverse effect on the pyridinoline cross-links occurring in type I collagen.[45] Thus, irrigation with NaOCl results in a structurally compromised collagen in the root dentin. Collagen is essential for the adhesion of epoxy resin sealer, as the latter has been shown to chemically bond to the amino groups of dentin.[46] This adhesion mechanism is hindered when NaOCl is used as a final rinse before the use of AH Plus in the canal. Another logical reason is that the oxygen bubbles formed following the use of NaOCl prevent the penetration of the epoxy resin sealer into the fine apertures of dentinal tubules.[47] These could be the reasons behind the lower bond strength in Group 1a. This finding was further confirmed by the increased number of adhesive failures at the dentin-sealer interface in Group 1a compared to other groups.
However, the results of this study contrast with another study by Donnenmayer et al. where maximum PBS values were exhibited by AH Plus as compared to BioRoot RCS and GuttaFlow 2 using different final irrigation protocols[48].
Irrigation with 6.5% PA significantly improved the PBS of AH Plus and BioRoot RCS to dentin. Studies have proven that PAs have excellent radical scavenging and antioxidant potential.[49] The multiple electron donor sites of PAs donate hydrogen atoms, thereby binding free radical molecules.[49] This property is directly proportional to the content of flavanols and their degree of polymerization.[50] The pH of GSE solution used in this study is 6. This slightly acidic pH could have also improved the bond strength of AH Plus and BioRoot RCS to dentin. These could be the reasons behind the enhanced bond strength seen in specimens irrigated with PA. The results are in accordance with several other studies.[51]
Previous studies have shown that significantly higher bond strength is recorded at the coronal and middle thirds of the canal compared to the apical third.[52] Such a pattern was observed only in specimens irrigated with PAs. In control and BS groups, no significant difference could be observed in intragroup comparisons. Hence, the null hypothesis stands rejected, as both the antioxidant applied and the varying regions of the canal influence the PBS of the sealer to dentin.
The results of the current study showed that no significant difference existed between PA-and BS-treated specimens. BS was as efficacious as PA in reinstating the reduced bond strength of AH Plus and BioRoot RCS to NaOCl-treated dentin. This could be attributed to the antioxidant potential of BS. Om and Jeong studied the superoxide dismutase activity of BS and inferred that BS was a potent antioxidant and it inhibited ROS formation. The efficacy of BS was found to be 4.5–7.2 times higher than table salt and 3.3–7.1 times higher than Vitamin E, a known antioxidant.[30]
The results correlated well with fracture analysis, with the fractured specimens in Group 1b, 1c, 2a, 2b, and 2c showing no adhesive failures and predominantly mixed failures compared to Group 1a.
BS and PA prove to be effective in bonding an epoxy resin sealer AH Plus and calcium silicate sealer BioRoot RCS to dentin irrigated with NaOCl. Future studies should also aim at the ultramorphological study of root surface irrigated with BS.
Financial support and sponsorship
Nil.
Conflicts of interest
There are no conflicts of interest.
| References | | |
| 1. | DeLong C, He J, Woodmansey KF. The effect of obturation technique on thepush-out bond strength of calcium silicate sealers. J Endod 2015;41:385-8.  |
| 2. | Barbizam JV, Trope M, Tanomaru-Filho M, Teixeira EC, Teixeira FB. Bond strength of different endodontic sealers to dentin: Push-out test. J Appl Oral Sci 2011;19:644-7. |
| 3. | Ray HA, Trope M. Periapical status of endodontically treated teeth in relation to the technical quality of the root filling and the coronal restoration. Int Endod J 1995;28:12-8. |
| 4. | Zehnder M. Root canal irrigants. J Endod 2006;32:389-98. |
| 5. | Zhu WC, Gyamfi J, Niu LN, Schoeffel GJ, Liu SY, Santarcangelo F, et al. Anatomy of sodium hypochlorite accidents involving facial ecchymosis – A review. J Dent 2013;41:935-48. |
| 6. | Moreira DM, Almeida JF, Ferraz CC, Gomes BP, Line SR, Zaia AA. Structural analysis of bovine root dentin after use of different endodontics auxiliary chemical substances. J Endod 2009;35:1023-7. |
| 7. | Mai S, Kim YK, Arola DD, Gu LS, Kim JR, Pashley DH, et al. Differential aggressiveness of ethylenediamine tetraacetic acid in causing canal wall erosion in the presence of sodium hypochlorite. J Dent 2010;38:201-6. |
| 8. | Cecchin D, Farina AP, Souza MA, Albarello LL, Schneider AP, Vidal CM, et al. Evaluation of antimicrobial effectiveness and dentine mechanical properties after use of chemical and natural auxiliary irrigants. J Dent 2015;43:695-702. |
| 9. | Versiani MA, Carvalho-Junior JR, Padilha MI, Lacey S, Pascon EA, Sousa-Neto MD. A comparative study of physicochemical properties of AH Plus and Epiphany root canal sealants. Int Endod J 2006;39:464-71. |
| 10. | Fisher MA, Berzins DW, Bahcall JK. An in vitro comparison of bond strength of various obturation materials to root canal dentin using a push-out test design. J Endod 2007;33:856-8. |
| 11. | Lai SC, Mak YF, Cheung GS, Osorio R, Toledano M, Carvalho RM, et al. Reversal of compromised bonding to oxidized etched dentin. J Dent Res 2001;80:1919-24. |
| 12. | Morris MD, Lee KW, Agee KA, Bouillaguet S, Pashley DH. Effects of sodium hypochlorite and RC-prep on bond strengths of resin cement to endodontic surfaces. J Endod 2001;27:753-7. |
| 13. | Farina AP, Cecchin D, Barbizam JV, Carlini-Júnior B. Influence of endodontic irrigants on bond strength of a self-etching adhesive. Aust Endod J 2011;37:26-30. |
| 14. | Manimaran VS, Srinivasulu S, Rajesh Ebenezar A, Mahalaxmi S, Srinivasan N. Application of a proanthocyanidin agent to improve the bond strength of root dentin treated with sodium hypochlorite. J Conserv Dent 2011;14:306-8. [ PUBMED] [Full text] |
| 15. | Vilanova WV, Carvalho-Junior JR, Alfredo E, Sousa-Neto MD, Silva-Sousa YT. Effect of intracanal irrigants on the bond strength of epoxy resin-based and methacrylate resin-based sealers to root canal walls. Int Endod J 2012;45:42-8. |
| 16. | Camps J, Jeanneau C, El Ayachi I, Laurent P, About I. Bioactivity of a calcium silicate-based endodontic cement (BioRoot RCS): Interactions with human periodontal ligament cells in vitro. J Endod 2015;41:1469-73. |
| 17. | Sarkar NK, Caicedo R, Ritwik P, Moiseyeva R, Kawashima I. Physicochemical basis of the biologic properties of mineral trioxide aggregate. J Endod 2005;31:97-100. |
| 18. | Prüllage RK, Urban K, Schäfer E, Dammaschke T. Material properties of a tricalcium silicate-containing, a mineral trioxide aggregate-containing, and an epoxy resin-based root canal sealer. J Endod 2016;42:1784-8. |
| 19. | Atmeh AR, Chong EZ, Richard G, Festy F, Watson TF. Dentin-cement interfacial interaction: Calcium silicates and polyalkenoates. J Dent Res 2012;91:454-9. |
| 20. | Watson TF, Atmeh AR, Sajini S, Cook RJ, Festy F. Present and future of glass-ionomers and calcium-silicate cements as bioactive materials in dentistry: Biophotonics-based interfacial analyses in health and disease. Dent Mater 2014;30:50-61. |
| 21. | Pimentel Corrêa AC, Cecchin D, de Almeida JF, Gomes BP, Zaia AA, Ferraz CC. Sodium thiosulfate for recovery of bond strength to dentin treated with sodium hypochlorite. J Endod 2016;42:284-8. |
| 22. | Castellan CS, Bedran-Russo AK, Karol S, Pereira PN. Long-term stability of dentin matrix following treatment with various natural collagen cross-linkers. J Mech Behav Biomed Mater 2011;4:1343-50. |
| 23. | Bedran-Russo AK, Pauli GF, Chen SN, McAlpine J, Castellan CS, Phansalkar RS, et al. Dentin biomodification: Strategies, renewable resources and clinical applications. Dent Mater 2014;30:62-76. |
| 24. | Nam JW, Phansalkar RS, Lankin DC, Bisson J, McAlpine JB, Leme AA, et al. Subtle chemical shifts explain the NMR fingerprints of oligomeric proanthocyanidins with high dentin biomodification potency. J Org Chem 2015;80:7495-507. |
| 25. | Berger SB, De Souza Carreira RP, Guiraldo RD, Lopes MB, Pavan S, Giannini M, et al. Can green tea be used to reverse compromised bond strength after bleaching? Eur J Oral Sci 2013;121:377-81. |
| 26. | Fine AM. Oligomeric proanthocyanidin complexes: History, structure, and phytopharmaceutical applications. Altern Med Rev 2000;5:144-51. |
| 27. | Hu M, McClements DJ, Decker EA. Antioxidant activity of a proanthocyanidin-rich extract from grape seed in whey protein isolate stabilized algae oil-in-water emulsions. J Agric Food Chem 2004;52:5272-6. |
| 28. | Hwang IM, Yang JS, Kim SH, Jamila N, Khan N, Kim KS, et al. Elemental analysis of sea, rock, and bamboo salts by inductively coupled plasma-optical emission and mass spectrometry. Anal Lett 2016;49:2807-21. |
| 29. | Moon JH, Shin HA, Rha YA, Om AS. The intrinsic antimicrobial activity of bamboo salt against Salmonella enteritidis. Mol Cell Toxicol 2009;5:323-7. |
| 30. | Om AS, Jeong JH. Bamboo salts have antioxidant activity and inhibit ROS formation in human astrocyte U373MG cells. J Cancer Prev 2007;12:225-30. |
| 31. | Zhao X, Jung OS, Park KY. Alkaline and antioxidant effects of bamboo salt. J Korean Soc Food Sci Nutr 2012;41:1301-4. |
| 32. | Lee HJ, Choi CH. Anti-inflammatory effects of bamboo salt and sodium fluoride in human gingival fibroblasts – An in vitro study. Kaohsiung J Med Sci 2015;31:303-8. |
| 33. | Kumar PS, Meganathan A, Shriram S, Sampath V, Sekar M. Effect of proanthocyanidin and bamboo salt on the push-out bond strength of an epoxy resin sealer to sodium hypochlorite-treated root dentin: An in vitro study. J Conserv Dent 2019;22:144-8. |
| 34. | Jainaen A, Palamara JE, Messer HH. Push-out bond strengths of the dentine-sealer interface with and without a main cone. Int Endod J 2007;40:882-90. |
| 35. | DeLong C, He J, Woodmansey KF. The effect of obturation technique on the push-out bond strength of calcium silicate sealers. J Endod 2015;41:385-8. |
| 36. | Gurgel-Filho ED, Martins F. Comparative evaluation of push-out bond strength of a MTA based root canal sealer. Braz J Oral Sci 2014;13:114-7. |
| 37. | Setia P, Sikri VK, Sroa RB, Sidhu B. Apical sealing ability of two novel root canal sealers: An ex-vivo study. J Int Clin Dent Res Organ 2013;5:9-13. [Full text] |
| 38. | Shokouhinejad N, Hoseini A, Gorjestani H, Shamshiri AR. The effect of different irrigation protocols for smear layer removal on bond strength of a new bioceramic sealer. Iran Endod J 2013;8:10-3. |
| 39. | Drummond JL, Sakaguchi RL, Racean DC, Wozny J, Steinberg AD. Testing mode and surface treatment effects on dentin bonding. J Biomed Mater Res 1996;32:533-41. |
| 40. | Sudsangiam S, van Noort R. Do dentin bond strength tests serve a useful purpose? J Adhes Dent 1999;1:57-67. |
| 41. | Nikaido T, Takano Y, Sasafuchi Y, Burrow MF, Tagami J. Bond strengths to endodontically-treated teeth. Am J Dent 1999;12:177-80. |
| 42. | Neelakantan P, Subbarao C, Subbarao CV, De-Deus G, Zehnder M. The impact of root dentine conditioning on sealing ability and push-out bond strength of an epoxy resin root canal sealer. Int Endod J 2011;44:491-8. |
| 43. | Weston CH, Ito S, Wadgaonkar B, Pashley DH. Effects of time and concentration of sodium ascorbate on reversal of NaOCl-induced reduction in bond strengths. J Endod 2007;33:879-81. |
| 44. | Estrela C, Estrela CR, Barbin EL, Spanó JC, Marchesan MA, Pécora JD. Mechanism of action of sodium hypochlorite. Braz Dent J 2002;13:113-7. |
| 45. | Daumer KM, Khan AU, Steinbeck MJ. Chlorination of pyridinium compounds. Possible role of hypochlorite, N-chloramines, and chlorine in the oxidation of pyridinoline cross-links of articular cartilage collagen Type II during acute inflammation. J Biol Chem 2000;275:34681-92. |
| 46. | Neelakantan P, Sharma S, Shemesh H, Wesselink PR. Influence of irrigation sequence on the adhesion of root canal sealers to dentin: A Fourier transform infrared spectroscopy and push-out bond strength analysis. J Endod 2015;41:1108-11. |
| 47. | Rocha AW, de Andrade CD, Leitune VC, Collares FM, Samuel SM, Grecca FS, et al. Influence of endodontic irrigants on resin sealer bond strength to radicular dentin. Bull Tokyo Dent Coll 2012;53:1-7. |
| 48. | Donnermeyer D, Vahdat-Pajouh N, Schäfer E, Dammaschke T. Influence of the final irrigation solution on the push-out bond strength of calcium silicate-based, epoxy resin-based and silicone-based endodontic sealers. Odontology 2019;107:231-6. |
| 49. | Vidhya S, Srinivasulu S, Sujatha M, Mahalaxmi S. Effect of grape seed extract on the bond strength of bleached enamel. Oper Dent 2011;36:433-8. |
| 50. | Yamaguchi F, Yoshimura Y, Nakazawa H, Ariga T. Free radical scavenging activity of grape seed extract and antioxidants by electron spin resonance spectrometry in an H(2)O(2)/NaOH/DMSO system. J Agric Food Chem 1999;47:2544-8. |
| 51. | Kalra M, Iqbal K, Nitisusanta LI, Daood U, Sum CP, Fawzy AS. The effect of proanthocyanidins on the bond strength and durability of resin sealer to root dentine. Int Endod J 2013;46:169-78. |
| 52. | Reddy P, Neelakantan P, Sanjeev K, Matinlinna JP. Effect of irrigant neutralizing reducing agents on the compromised dislocation resistance of an epoxy resin and a methacrylate resin-based root canal sealer in vitro. Int J Adhes Adhes 2018;82:206-10. |
Correspondence Address:
Dr. Kaur Kamalpreet
Department of Conservative Dentistry and Endodontics, National Dental College and Hospital, Derabassi, Punjab
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/jcd.jcd_52_21
[Table 1], [Table 2] |
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