| ORIGINAL ARTICLE |
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| Year : 2021 | Volume
: 24
| Issue : 1 | Page : 63-66 |
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Evaluation of surface analysis of gutta-percha after disinfecting with sodium hypochlorite, silver nanoparticles, and chitosan nanoparticles by atomic force microscopy: An in vitro study
P Karunakar, MS Ranga Reddy, Umrana Faizuddin, Basa Srinivas Karteek, Chavva Lakshmi Charan Reddy, Marupaka Rasagna
Department of Conservative Dentistry and Endodontics, Panineeya Institute of Dental Sciences and Research Centre, Hyderabad, Telangana, India
Correspondence Address:
Dr. Chavva Lakshmi Charan Reddy
Plot No 18 East, First Floor, Self Finance Colony, Vanasthalipuram, Hyderabad, Telangana
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/JCD.JCD_505_20
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Aims and Objectives: The purpose of this study is to evaluate the surface topography of gutta-percha (GP) after disinfecting with sodium hypochlorite (NaOCl) (5.25%), silver nanoparticles (AgNPs) (70 μg/ml), and chitosan nanoparticles (ChNPs) (1.5 mg/ml) by atomic force microscopy (AFM).
Materials and Methods: Forty GP cones were taken in this in vitro study. These samples were divided into four different groups such as Group I – control group (untreated GP Points) and Group II, III, and IV were treated with 5.25% NaOCl, 70 μg/ml AgNPs, and 1.5 mg/ml ChNPs, respectively. The surface topography analysis of the samples was performed using AFM.
Statistical Analysis: Root mean square (RMS) and surface roughness parameters were used to compare the structure of GP points with contact mode imaging. These values were tested by IBM SPSS-20.0 version statistical software using one-way ANOVA and post hoc (Tukey's honestly significant difference) tests. They were considered statistically significant when P < 0.05.
Results: The RMS and surface roughness values are significantly higher for NaOCl group (5.25% NaOCl) when compared with AgNPs group (70 μg/ml AgNPs) and ChNPs group (1.5 mg/ml ChNPs).
Conclusion: This study has shown more surface topography deterioration of GP treated with NaOCl and lesser deterioration with AgNPs and ChNPs which affects postoperative prognosis.
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