Journal of Conservative Dentistry
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Year : 2020  |  Volume : 23  |  Issue : 3  |  Page : 270-274

Assessment of various endodontic instrumentation systems on the amount of apically extruded bacteria - An in vitro study

1 Department of Conservative Dentistry and Endodontics, Bhojia Dental College and Hospital, Baddi, Himachal Pradesh, India
2 Department of Conservative Dentistry and Endodontics, Rayat Bahra Dental College and Hospital, Mohali, Punjab, India
3 Department of Oral and Maxillofacial Surgery, Dr. Harvansh Singh Judge Institute of Dental Sciences and Hospital, Chandigarh, India

Correspondence Address:
Dr, Ajay Chhabra
5000, Ground Floor, Sector 38 West, Chandigarh - 160 036
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/JCD.JCD_298_20

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Aim: The objective of this in vitro study was to assess the effect of different endodontic instrumentation systems on the amount of apically extruded bacteria. Materials and Methods: One hundred and twenty freshly extracted human mandibular premolars with single canal were collected. Endodontic access cavities were prepared and then contaminated with an Enterococcus faecalis suspension (ATCC 29212). After incubation at 37°C for 24 h, the root canals were instrumented with K flare files, F360 Single file system, K3XF files, Heroshaper files, Protaper Next files, and Hyflex EDM Single file system. During instrumentation, apically extruded bacteria were collected in the vials containing 0.9% NaCl. Samples were taken from the vials and incubated in brain–heart infusion agar medium for 24 h. Statistical Analysis: The number of colony-forming units was determined, and data were statistically analyzed using one-way analysis of variance and post hoc Tukey test. Results: Significant difference was found between the rotary and hand instrumentation systems. Conclusions: Both rotary and hand instrumentation systems extruded intracanal bacteria through the apical foramen, Group 1 (Hand K Flare files) showed maximum, whereas, Group 5 (Protaper Next) and Group 6 (Hyflex EDM) showed the least extrusion.

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