| ORIGINAL ARTICLE |
|
| Year : 2015 | Volume
: 18
| Issue : 6 | Page : 474-478 |
|
|
Antimicrobial efficacy of different root canal sealers by using real-time polymerase chain reaction: An ex vivo study
R Gnana Seelan, A Arvind Kumar, R Jonathan Emil Sam, S Uma Maheswari
Department of Conservative Dentistry and Endodontics, Rajas Dental College and Hospital, Tirunelveli, Tamil Nadu, India
Correspondence Address:
Dr. S Uma Maheswari
Department of Conservative Dentistry and Endodontics, Rajas Dental College and Hospital, Raja Nagar, Kavalkinaru, Tirunelveli, Tamil Nadu
India
 Source of Support: None, Conflict of Interest: None  | Check |
DOI: 10.4103/0972-0707.168814
|
|
|
Background: Root canal sealers help to minimize leakage, provides antimicrobial activity to reduce the possibility of residual bacteria, and to resolve periapical lesion.
Aim: To compare five different root canal sealers against Enterococcus faecalis in an infected root canal model by using real-time polymerase chain reaction (RT-PCR).
Settings and Design: Sixty human mandibular premolars were sectioned to standardize a uniform length of 14 mm. Fifty microliters of the inoculum containing E. faecalis were transferred into each microcentrifuge tube (n = 60). The samples were divided into six groups Tubli-Seal, Apexit Plus, Fillapex, AH Plus, RoekoSeal, and Positive control, respectively.
Materials and Methods: Five groups after the incubation with the microorganism E. faecalis were coated with different root canal sealers and obturated using F3 ProTaper Gutta-percha point. The dentinal shavings were collected and analyzed for RT-PCR.
Statistical Analysis: The mean difference between six groups was calculated using analysis of variance and post-hoc test.
Results: The highest antibacterial activity was achieved with Tubli-Seal (1938.13 DNA in pictogram [pg]) and least by RoekoSeal (3034.54 DNA in pg).
Conclusion: The maximum antimicrobial activity was achieved AH Plus and Tubli-Seal. RT-PCR can be used as a valuable and accurate tool for testing antimicrobial activity. |
|
|
|
| [FULL TEXT] [PDF]* |
|
 |
|
