Antibacterial efficacy of Azadirachta indica, Mimusops elengi and 2% CHX on multispecies dentinal biofilm
Kunjal S Mistry1, Zarna Sanghvi2, Girish Parmar3, Samir Shah4, Kasukurthi Pushpalatha5
1 Department of Conservative Dentistry and Endodontics, Faculty of Dental Science, Dharamsinh Desai University, Nadiad, Gujarat, India
2 Department of Conservative Dentistry and Endodontics, Ahmedabad Dental College and Hospital, Ahmedabad, Gujarat, India
3 Department of Conservative Dentistry and Endodontics, Government Dental College and Hospital, Ahmedabad, Gujarat, India
4 Department of Pharmacology, Sardar Patel College of Pharmacy, Anand, Gujarat, India
5 Senior Research Associate, Disha Life Science, Ahmedabad, Gujarat, India
Dr. Kunjal S Mistry
Sharnam Dental Clinic, Office No. 101, Shailly Complex, Surdhara Circle, SAL Hosptial Road, Thaltej, Ahmedabad - 380054, Gujarat
Source of Support: None, Conflict of Interest: None
Aims: To check the antimicrobial activity of Azadirachta indica (Neem), Mimusops elengi (Bakul), and Chlorhexidine gluconate (CHX) on multispecies biofilm of common endodontic pathogens such as Streptococcus mutans, Enterococcus faecalis, Staphylococcus aureus and Candida albicans.
Settings and Design: In vitro dentin disinfection model used to check the antimicrobial efficacy of herbal extracts.
Materials and Methods: The in vitro dentin disinfection model was used to check the antimicrobial activity of the methanolic extracts of the medicinal plants along with Chlorhexidine gluconate. The polymicrobial biofilm was allowed to grow on extracted teeth sections for a period of 21 days. Remaining microbial load in the form of CFU/ml after the antimicrobial treatment was tabulated, and data were statistically analyzed using ANOVA and Bonferroni post-hoc tests.
Statistical Analysis Used: SPSS version 17, one-way ANOVA, Bonferroni post-hoc test.
Results: Both the plant extracts showed considerable antimicrobial efficacy as compared to negative control. 2% CHX was the most effective antimicrobial agent having statistically significant difference against plant extracts and negative control (saline).
Conclusion: The methanolic extract of A. Indica, M. elengi, and Chlorhexidine Gluconate has considerable antimicrobial activity against polymicrobial dentinal biofilm of S. mutans, E. faecalis, S. aureus and C. albicans.