Journal of Conservative Dentistry
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Year : 2015  |  Volume : 18  |  Issue : 1  |  Page : 51-55

Effect of epigallocatechin-3-gallate application for remaining carious dentin disinfection

Faculty of Pharmacy, Dentistry and Nursing, Department of Restorative Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil

Correspondence Address:
Prof. Sérgio Lima Santiago
Rua Monsenhor Furtado, s/n Rodolfo Teófilo, 60.430-355 Fortaleza-CE
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Source of Support: None, Conflict of Interest: None

DOI: 10.4103/0972-0707.148896

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Context: Epigallocatechin-3-gallate (EGCG) is a flavonoid extracted from green tea that demonstrated antimicrobial activity. Aims: To evaluate the efficacy of EGCG 0.5%, 1%, and 2% concentrations as an antimicrobial solution in dentin caries-like lesions induced in a bacterial-based in vitro model. Materials and Methods: Twenty-five human dentin specimens were submitted to a microbial-based caries model by immersion in brain heart infusion (BHI) broth inoculated with Streptococcus mutans UA159, for 5 days. After the demineralization period, the specimens were randomly divided into groups: Group I: 0.9% saline solution; Group II: 2% chlorhexidine digluconate; Group III: 0.5% EGCG; Group IV: 1% EGCG; and Group V: 2% EGCG. After the treatments, carious dentin samples were harvested from dentin specimens and analyzed by colony-forming unit (CFU) counts. Data were analyzed by ANOVA and Tukey's test. Results: Log reduction values (SD, -1 ) for Groups I-V were: 5.02 (0.16), 3.96 (0.43), 4.74 (0.26), 4.89 (0.56), and 4.91 (0.40), respectively. There was no statistical difference between the EGCG concentrations and saline solution (P > 0.05). Furthermore, there was no statistical difference between EGCG concentrations (P > 0.05). However, there was a statistically significant difference between the chlorhexidine digluconate group and the other groups (P < 0.05). Conclusion: EGCG at the studied concentrations were not effective in eliminating S. mutans from dentin caries-like lesions.

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