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30 maxillary/mandibular anterior teeth with apical periodontitis were selected. The age factor varied between 15-40 years. Cases were divided into three groups on the basis of the combination of symptoms. Access openings were prepared and sterile paper points were inserted into the canals for one minute and then were immediately inoculated in thioglycollate broth which were later incubated aerobically as well as anaerobically . Each culture plate were examined and different aerobic and anaerobic bacteria were isolated and identified. The number of anaerobes was greater than aerobes in infected root canals. Peptostreptococcus, Peptococcus, Eubacterium , Porphyromonas and Bacteroides were commonly isolated in cases having pain on percussion. Porphyromonas and Bacteriodes were significantly related to odour in infected root canal. Peptostreptococcus, peptococcus, and Porphyromonas gingivalis were found to be significantly related to subacute clinical symptoms.
