Journal of Conservative Dentistry

ORIGINAL ARTICLE
Year
: 2016  |  Volume : 19  |  Issue : 6  |  Page : 522--526

Cytotoxicity of two available mineral trioxide aggregate cements and a new formulation on human gingival fibroblasts


Maryam Torshabi1, Reza Amid2, Mahdi Kadkhodazadeh2, Sara Eslami Shahrbabaki2, Fahimeh S Tabatabaei1 
1 Department of Dental Biomaterials, School of Dentistry; Department of Tissue Engineering, School of Advanced Technologies in Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2 Department of Periodontics, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Correspondence Address:
Fahimeh S Tabatabaei
Department of Dental Biomaterials, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran
Iran

Aim: The purpose of this study was to investigate the cytotoxicity of nanohybrid mineral trioxide aggregate (MTA) in comparison with calcium-enriched mixture (CEM) cement and MTA-Angelus, using human gingival fibroblasts (HGFs). Materials and Methods: Nine disc-shaped specimens of each material (in 2 set stat: A, set for 24 h; B, set for 30 min; and C, fresh stat) were prepared. HGFs were exposed to tested materials' extracts or control media. Cytotoxicity testing was performed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay in two time intervals. Statistical Analysis: Results were evaluated by one-way ANOVA and t-test. Statistical significance was set at P< 0.05. Results: CEM cement demonstrated favorable cell viability values when completely set (24 h set MTA = 24 h set CEM) at both time intervals. Interestingly, 24 h after incubation, CEM in Groups B and C demonstrated higher cell viability values than MTA (P < 0.05). However, after 72 h of incubation, these groups of CEM and MTA showed equal cell viability. All samples of nanohybrid MTA had slight cytotoxic effects after 24 h of incubation, and moderate cytotoxic effects after 72 h of incubation. Conclusion: Set CEM and set MTA-Angelus exerted similar, favorable effects on cell viability. However, within the limitations of this in vitro study, the results suggest that nanohybrid MTA could not be recommended as a material of choice for cervical root resorption.


How to cite this article:
Torshabi M, Amid R, Kadkhodazadeh M, Shahrbabaki SE, Tabatabaei FS. Cytotoxicity of two available mineral trioxide aggregate cements and a new formulation on human gingival fibroblasts.J Conserv Dent 2016;19:522-526


How to cite this URL:
Torshabi M, Amid R, Kadkhodazadeh M, Shahrbabaki SE, Tabatabaei FS. Cytotoxicity of two available mineral trioxide aggregate cements and a new formulation on human gingival fibroblasts. J Conserv Dent [serial online] 2016 [cited 2017 Feb 21 ];19:522-526
Available from: http://www.jcd.org.in/article.asp?issn=0972-0707;year=2016;volume=19;issue=6;spage=522;epage=526;aulast=Torshabi;type=0